abstract：:The quantitation of serum levels of hepatitis C virus (HCV) RNA in chronic hepatitis C has been regarded as one of the most important indicators for the outcome of interferon (IFN) therapy. The AMPLICOR HCV MONITOR version 1.0 (AMPLICOR v1.0) assay is widely used for the evaluation of the HCV level. A new generation a...

journal_title：Journal of medical virology

authors： Kawai S,Yokosuka O,Imazeki F,Saisho H,Mizuno C

更新日期：2002-11-01 00:00:00

abstract：:The aim of the study was to assess the prevalence of human papillomavirus (HPV) in the esophagus in the coastal region of Eastern Guangdong, Southern China, an area with a high incidence of esophageal carcinoma. Fresh surgical resection esophageal specimens were obtained from 176 esophageal carcinoma patients admitted...

journal_title：Journal of medical virology

authors： Shen ZY,Hu SP,Lu LC,Tang CZ,Kuang ZS,Zhong SP,Zeng Y

更新日期：2002-11-01 00:00:00

abstract：:The objective of the study was to assess the genotypic and phenotypic properties of 18 viral strains from human immunodeficiency virus-1 (HIV-1) positive patients and to identify subtype C isolates for vaccine design strategies. All the isolates were non-syncytium-inducing (NSI) in both the primary and MT-2 cell cultu...

journal_title：Journal of medical virology

authors： Treurnicht FK,Smith TL,Engelbrecht S,Claassen M,Robson BA,Zeier M,van Rensburg EJ

更新日期：2002-10-01 00:00:00

abstract：:Although occult hepatitis B virus (HBV) infection in individuals without detectable hepatitis B surface antigen (HBsAg) may occur and has been reported to be common in patients with chronic hepatitis C, the related molecular mechanisms remain unknown. With the polymerase chain reaction, serum HBV DNA was sought in 100...

journal_title：Journal of medical virology

authors： Kao JH,Chen PJ,Lai MY,Chen DS

更新日期：2002-10-01 00:00:00

abstract：:Amphotropic murine leukemia virus (MLV) replicates in cells from various mammalian species including humans and is a potential contaminant in MLV vector preparations for human gene transfer studies. In general, MLV replication depends on the expression of viral genes under the control of 75 bp enhancer elements in the...

journal_title：Journal of medical virology

authors： Reuss FU,Berdel B,Heber R,Bantel-Schaal U

更新日期：2002-10-01 00:00:00

abstract：:Virus variants escaping from host immunity may be implicated in the pathogenesis of hepatitis B virus (HBV) infection. In this cross-sectional study, the association was evaluated of the frequency of amino acid variation within the immunogenic epitopes of surface gene with different disease stages of chronic HBV infec...

journal_title：Journal of medical virology

authors： Liu CJ,Kao JH,Shau WY,Chen PJ,Lai MY,Chen DS

更新日期：2002-09-01 00:00:00

abstract：:Simian immunodeficiency virus (SIV) as well as human immunodeficiency virus (HIV) induce polyclonal B-cell activation and are associated with the appearance of lymphomas in their respective hosts in either the presence or the absence of other co-infecting viruses such as Epstein-Barr virus (EBV). However, the pathogen...

journal_title：Journal of medical virology

authors： Titti F,Zamarchi R,Maggiorella MT,Sernicola L,Geraci A,Negri DR,Borsetti A,Menin C,D'Andrea E,Modesti A,Masuelli L,Verani P,Chieco-Bianchi L,Amadori A

更新日期：2002-09-01 00:00:00

abstract：:Fifty-three (96%) of 55 patients with coronary artery stenosis were positive for serum anti-HHV-6 IgG, and 50 (91%) of these patients had anti-HHV-7 IgG. The number of cases sero-positive for HHV-6 and -7 in the 54 age matched control volunteers was 52 (96%) and 53 (98%), respectively. No statistical difference in the...

journal_title：Journal of medical virology

authors： Ihira M,Yoshikawa T,Ishii J,Nomura M,Hishida H,Ohashi M,Enomoto Y,Suga S,Iida K,Saito Y,Nishiyama Y,Asano Y

更新日期：2002-08-01 00:00:00

abstract：:The purpose of this study was to evaluate the use of dried blood spots stored on filter paper as a means to provide specimens for virologic surveillance for measles virus (MV) in situations when the reverse cold chain is not available. Two single-step RT-PCR assays were evaluated for sensitivity of detection of MV nuc...

journal_title：Journal of medical virology

authors： Katz RS,Premenko-Lanier M,McChesney MB,Rota PA,Bellini WJ

更新日期：2002-08-01 00:00:00

abstract：:In the nine years since the Global Advisory Group of the Expanded Programme on Immunisation (WHO) set 1997 as the target for integrating hepatitis B vaccination into national immunisation programmes worldwide, 129 countries have included hepatitis B vaccine as part of their routine infant or adolescent immunisation pr...

journal_title：Journal of medical virology

authors： Van Damme P,Vorsters A

更新日期：2002-07-01 00:00:00

abstract：:An "in-house" recombinant virus protease inhibitor susceptibility assay was carried out (median of 3 per patient) retrospectively in 26 patients failing HIV protease inhibitor based therapy at regular intervals to the initiation of the first protease inhibitor. Patients were treated with either indinavir (N = 6), rito...

journal_title：Journal of medical virology

authors： Servais J,Plesséria JM,Lambert C,Fontaine E,Robert I,Arendt V,Staub T,Schneider F,Hemmer R,Schmit JC

更新日期：2002-07-01 00:00:00

abstract：:A reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of Chikungunya virus infection. Based on the nonstructural protein 1 (nsP1) and glycoprotein E1 (E1) genes of Chikungunya, two primer sets were designed. Total RNA were extracted from the cell culture fluid of Aedes albopictus C...

journal_title：Journal of medical virology

authors： Hasebe F,Parquet MC,Pandey BD,Mathenge EG,Morita K,Balasubramaniam V,Saat Z,Yusop A,Sinniah M,Natkunam S,Igarashi A

更新日期：2002-07-01 00:00:00

abstract：:The human cytomegalovirus (HCMV), a member of the Herpesviridae, is the most frequent cause of congenital virus infections and a major cause of morbidity and mortality in immunocompromised patients. Due to the lack of an appropriate animal model, insight into the pathogenesis of HCMV infections originates primarily fr...

journal_title：Journal of medical virology

authors： Bissinger AL,Sinzger C,Kaiserling E,Jahn G

更新日期：2002-06-01 00:00:00

abstract：:A real-time PCR assay was developed for quantitative detection of B19 DNA in clinical serum samples. The assay was carried out using a LightCycler instrument and product formation was monitored continuously with the fluorescent double-stranded DNA binding dye SYBR Green I. With an optimized PCR protocol, this system w...

journal_title：Journal of medical virology

authors： Manaresi E,Gallinella G,Zuffi E,Bonvicini F,Zerbini M,Musiani M

更新日期：2002-06-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) and human immunodeficiency virus type 1 (HIV-1) may interact during transplacental transmission of HIV-1. The placental syncytiotrophoblast layer serves as the first line of defense of the fetus against viruses. Patterns of replication of HHV-6 variant A (HHV-6A) and HIV-1 were analyzed in ...

journal_title：Journal of medical virology

authors： Csoma E,Bácsi A,Liu X,Szabó J,Ebbesen P,Beck Z,Kónya J,Andirkó I,Nagy E,Tóth FD

更新日期：2002-05-01 00:00:00

abstract：:Hepatitis B e antigen (HBeAg)-negative chronic hepatitis B results from infection with hepatitis B virus mutants unable to produce HBeAg. It accounts for 7-30% of patients with chronic hepatitis B virus (HBV) worldwide, with the highest rates reported for Mediterranean Europe and Asia. Interferon (IFN) is currently th...

journal_title：Journal of medical virology

authors： Rizzetto M

更新日期：2002-04-01 00:00:00

abstract：:A multiplex polymerase chain reaction (PCR) assay that is capable of detecting and typing six serotypes of respiratory adenovirus (Ad) was developed, using multiple sets of type-specific primers. The detection of each different serotype depended on distinguishing different numbers and sizes of amplification products o...

journal_title：Journal of medical virology

authors： Na BK,Kim JH,Shin GC,Lee JY,Lee JS,Kang C,Kim WJ

更新日期：2002-04-01 00:00:00

abstract：:There are contradictory reports regarding the effects of inhalation anesthetics on the immune system. Measurable immune responses have been studied in vitro, but little is known about the in vivo effects in the intact organism. We used an attenuated, non-neuroinvasive, nonlethal strain of the encephalitic West Nile vi...

journal_title：Journal of medical virology

authors： Katz Y,Lustig S,Ben-Shlomo I,Kobiler D,Ben-Nathan D

更新日期：2002-04-01 00:00:00

abstract：:It has been reported that a new pattern of mutations, E44D/A and/or V118I, in the reverse transcriptase (RT) gene of HIV-1 confers a moderate level of resistance to lamivudine in the absence of the M184V mutation. The prevalence of this mutational pattern was studied in HIV-1 isolates obtained from 280 patients. These...

journal_title：Journal of medical virology

authors： Montes B,Segondy M

更新日期：2002-03-01 00:00:00

abstract：:Corticosteroid use in transplant recipients increases the incidence and severity of Kaposi's sarcoma (KS), a disease associated with KS-associated herpesvirus (KSHV) infection. Recently, the prototypic corticosteroid, hydrocortisone, was shown to directly induce lytic cycle reactivation of KSHV in latently-infected BC...

journal_title：Journal of medical virology

authors： Zoeteweij JP,Rinderknecht AS,Davis DA,Yarchoan R,Blauvelt A

更新日期：2002-03-01 00:00:00

abstract：:In vitro incubation of human cytomegalovirus (Towne strain) with 8 U/ml human recombinant myeloperoxidase plus sodium chloride and glucose nearly abolished viral infectivity. To assay the effect on intracellular infection, cell toxicity of the enzymes was first studied. Even the high dose of 16 U/ml of recombinant mye...

journal_title：Journal of medical virology

authors： El Messaoudi K,Verheyden AM,Thiry L,Fourez S,Tasiaux N,Bollen A,Moguilevsky N

更新日期：2002-02-01 00:00:00

abstract：:In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detecte...

journal_title：Journal of medical virology

authors： Yu Q,Shiramizu B,Nerurkar VR,Hu N,Shikuma CM,Melish ME,Cascio K,Imrie A,Lu Y,Yanagihara R

更新日期：2002-02-01 00:00:00

abstract：:Nasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharynge...

journal_title：Journal of medical virology

authors： Liu Y,Wang X,Lo AK,Wong YC,Cheung AL,Tsao SW

更新日期：2002-01-01 00:00:00

abstract：:Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show ...

journal_title：Journal of medical virology

authors： Moreli ML,Aquino VH,Cruz AC,Figueiredo LT

更新日期：2002-01-01 00:00:00

abstract：:Hepatitis C Virus (HCV) NS3 protease is an attractive target for antiviral agent development because it is required for viral replication. Because a stable cell culture system or small animal model to study HCV replication is not readily available, we constructed an in vitro model allowing the investigation of NS3 tra...

journal_title：Journal of medical virology

authors： Heintges T,Encke J,zu Putlitz J,Wands JR

更新日期：2001-12-01 00:00:00

abstract：:During the 1998-1999 influenza season, two distinct influenza B virus Yamagata group strains were isolated from the patients of a private clinic. Each responded differently to monoclonal antibodies (Mabs) 5H4 and 8B3 on staining, and hemagglutination inhibition and neutralizing tests. When the analysis of nucleotide s...

journal_title：Journal of medical virology

authors： Nakagawa N,Kubota R,Morikawa S,Nakagawa T,Baba K,Okuno Y

更新日期：2001-12-01 00:00:00

abstract：:Reactivation of hepatitis B e antigen (HBeAg) negative chronic hepatitis B virus (HBV) infection due to selection of precore variant virus is an uncommon complication of previous hepatitis B infection, and virtually unrecognised in children and adolescents. A child who had received treatment with methylprednisolone an...

journal_title：Journal of medical virology

authors： Smith PR,Zampino R,Gutteridge C,Karayiannis P,Aitken C

更新日期：2001-11-01 00:00:00

abstract：:A common receptor for coxsackie B virus and adenovirus has been described recently in cells of human and murine origin. Since the established cell line A-549 is suitable for adenoviruses, the potential use of A-549 cells for the isolation of coxsackie B viruses from clinical samples was investigated. All throat swabs ...

journal_title：Journal of medical virology

authors： Otero JR,Folgueira L,Trallero G,Prieto C,Maldonado S,Babiano MJ,Martinez-Alonso I

更新日期：2001-11-01 00:00:00

abstract：:Puumala hantavirus (PUUV) glycoproteins G1 and G2 and nucleocapsid protein (N) were expressed in BHK-21 cells by transfection of a plasmid producing a recombinant alphavirus replicon. Coexpression of G1 and G2 from separate constructs seemed to be important for the optimal folding of the glycoproteins, as evaluated by...

journal_title：Journal of medical virology

authors： Kallio-Kokko H,Leveelahti R,Brummer-Korvenkontio M,Lundkvist A,Vaheri A,Vapalahti O

更新日期：2001-11-01 00:00:00

abstract：:A sensitive and reproducible real-time PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an "in-house" qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B patients, consisting of 22 patients ...

journal_title：Journal of medical virology

authors： Chen RW,Piiparinen H,Seppänen M,Koskela P,Sarna S,Lappalainen M

更新日期：2001-10-01 00:00:00

abstract：:Dengue virus (DV) infection can result in either a mild febrile illness known as dengue fever (DF) or a life-threatening disease called dengue hemorrhagic fever (DHF). DHF is more prevalent in patients undergoing secondary DV infection. This observation has led to the hypothesis that DHF may be the result of immune re...

journal_title：Journal of medical virology

authors： Spain-Santana TA,Marglin S,Ennis FA,Rothman AL

更新日期：2001-10-01 00:00:00

abstract：:Recent studies have suggested a pathogenic role of human parvovirus B19 (B19) in the development of acute fulminant liver failure in children. The hypothesis was based on the detection of B19 DNA in 8 of 10 explanted livers of children requiring liver transplantation. In the present study, explanted livers from 43 adu...

journal_title：Journal of medical virology

authors： Eis-Hübinger AM,Reber U,Abdul-Nour T,Glatzel U,Lauschke H,Pütz U

更新日期：2001-10-01 00:00:00

abstract：:The purpose of this study was to investigate determinants of endemic hepatitis C virus (HCV) infection within communities in Taiwan. A two-phase study, including a seroprevalence survey and a prevalent case-control study at the first phase, which has been published previously, and a follow-up seroconversion determinat...

journal_title：Journal of medical virology

authors： Sun CA,Chen HC,Lu SN,Chen CJ,Lu CF,You SL,Lin SH

更新日期：2001-09-01 00:00:00

abstract：:Adhesion molecules are essential for the immune response. They are involved in the regulation of cell-to-cell contact, thereby enabling leukocytes to communicate. Circulating forms of adhesion molecules are found in the serum of healthy individuals. Raised levels have been associated with disease severity in HCV and o...

journal_title：Journal of medical virology

authors： Murgue B,Cassar O,Deparis X

更新日期：2001-09-01 00:00:00

abstract：:The immunogenicity of the Russian cold-adapted (ca) donor stains, A/Leningrad (Len)/134/17/57 and A/Leningrad/134/47/57, and the US strain A/Ann Arbor (AA)/6/60-ca, were compared in BALB/c mice with their respective wild-type parental viruses. Each ca donor strain was less immunogenic than its wild-type parent. The va...

journal_title：Journal of medical virology

authors： Wareing MD,Watson JM,Brooks MJ,Tannock GA

更新日期：2001-09-01 00:00:00

abstract：:One hundred fifty-five chronic hepatitis C patients were assigned at random to receive natural lymphoblastoid interferon (IFN)alpha-n1, s.c., for 13 months in one of three treatment regimens: initial daily induction with 10 million units (MU) followed (group 1, n = 50) or not (group 2, n = 52) by 1 month of rest and t...

journal_title：Journal of medical virology

authors： Diago M,Suárez D,García-Villarreal L,Castro A,Domínguez A,Pardo M,del Olmo JA,Pérez-Hernández F,Aguilar J,Quiroga JA,Carreño V

更新日期：2001-08-01 00:00:00

abstract：:The objectives of this study were to evaluate the reliability of herpes simplex virus (HSV) PCR testing in cerebrospinal fluid (CSF) for the detection of herpes simplex encephalitis. This was done by examining retrospectively the clinical follow-up of a large group of patients tested routinely by HSV-PCR. In addition,...

journal_title：Journal of medical virology

authors： Puchhammer-Stöckl E,Presterl E,Croÿ C,Aberle S,Popow-Kraupp T,Kundi M,Hofmann H,Wenninger U,Gödl I

更新日期：2001-08-01 00:00:00

abstract：:The ability of the anti-cancer drug, 9-Nitrocamptothecin (9NC), to inhibit replication of HIV-1 in clinically relevant primary lymphocytic cells was studied. Primary peripheral blood lymphocytes (PBLs) from a non-infected donor were freshly infected with HIV-1 and treated with 9NC by using three different treatment sc...

journal_title：Journal of medical virology

authors： Hung CL,Doniger J,Palini A,Snyder SW,Radonovich MF,Brady JN,Pantazis P,Sadaie MR

更新日期：2001-07-01 00:00:00

abstract：:Isolated reactivity to hepatitis B virus (HBV) core antigen (anti-HBc) is observed relatively frequently in immunocompromised individuals, intravenous drug abusers (IVDA), and in the presence of HCV infection. The reason for the lack of HBsAg is not clear. The aim of the present study was to investigate which factors ...

journal_title：Journal of medical virology

authors： Weber B,Melchior W,Gehrke R,Doerr HW,Berger A,Rabenau H

更新日期：2001-07-01 00:00:00

abstract：:Early estimates of the prevalence of TTV viremia in healthy adults of developed countries were in the order of 1--10 %, while similar estimates in Third World countries were considerably higher. Using three different PCRs, TTV-related DNA was detected in serum from 180 out of the 201 Norwegian blood donors tested, ind...

journal_title：Journal of medical virology

authors： Huang LY,Oystein Jonassen T,Hungnes O,Grinde B

更新日期：2001-07-01 00:00:00